Effect of Phenylhydrazine - induced Hemoly tic Anemia on Nuclear RNA Polymerase Activity

The DNA-dependent RNA polymerase of tenfold increase in the nuclear polymerase nuclei from lymphoid-rich and erythroidactivity per milligram nuclear DNA of the rich mouse spleens was compared in redeveloping erythropoietic spleen when the gard to requirements and conditions of the assays were done at low ionic strength reactions. The inhibition by either actinoand more than a threefold increase at high mycin D or pancreatic DNase indicated ionic strength. The peak rise in polymerase that a DNA template was required for the activity precedes the maximal developobserved reactions. Following the inducment of the erythropoietic spleen by 3 tion of a hemolytic anemia by the admindays. istration of phenylhydrazine, there was a